Endolitiska lavar. (Bryonet 070413) Dear Scott, endoliths are organisms which are able to form boreholes, furrows and channels within the substratum by mean of an active process of dissolution of the matrix (?euendoliths? sensu Golubic et al. 1981). For these peculiarities, they do differ from other organisms living within the rocks: the ?chasmolithics?, which simply adhere to cracks of the substrata, and the ?cryptoendoliths?, which colonise the structural holes of porous substrata (most ?endolithic? lichens from Antarctica belong here). Some endoliths are able to penetrate quartz crystals, but the heaviest colonisation typically occurs in carbonatic rocks, such as (in order of decreasing frequency) sedimentary and metamorphic limestone, dolomitic rocks and sandstone. Endolithic lichens are, together with cyanobacteria, the most common endoliths of European carbonatic outcrops (W. Pohl, in Schneider & le Campion-Alsumard 1999). The symbionts, but particularly the mycobiont, penetrate into the substratum to a depth of 2-3 mm (Pomar et al. 1975, Pinna et al. 1998). The colonisation of the rocks is often recognizable only by a light fading or pigmentation of the substratum (Doppelbaur 1959, Gerhmann-Janssen 1995, Gerhmann & Krumbein 1996, Pinna et al. 1998), and a careful observation is needed to detect the presence of their fruiting bodies. Surprisingly, the mechanisms used by endolithic organisms to dissolve the substratum are still unknown, although different hypothesis have been proposed (Pia 1937).These may be divided in two main categories, 1) biological corrosion, consisting in the chemical dissolution of the substratum, and 2) biological abrasion, consisting in the mechanical removal of carbonate (Golubic & Schneider 1979). In calcicolous endolithic lichens biological corrosion prevails (Pinna et al. 1998). As it is well known that the carbonatic matrix is easily attacked by acids (including weak acids), it was hypothesised that the penetration of endoliths might be related to the same factors that are at the basis of the chemical processes involved in the biodeterioration of any rock. These factors, that are nor mutually exclusive, are: 1) the secretion of organic acids, such as oxalic acid; 2) the secretion of chelating compounds (e.g. some lichen substances); 3) the production of carbonic acid derived from respiratory CO2 dissolution (Syers and Iskandar 1973). Although these processes have been described in many epilithic organisms, in our opinion there are not yet sufficient evidences supporting their role in calcicolous endolithic lichens. For this reason, our research groups have concentrated their efforts to identify the mechanism of calcite dissolution in endolithic lichens from the Trieste Karst (N Italy). Histological, biochemical and biomolecular techniques applied to thalli still immersed in the substratum, or free from it showed that all the species produce specific carbonic anhydrases (CA). CAs are particularly frequent in the lithocortex and in the oil-hyphae of the pseudomedulla (see Pinn et al. 1998 for tan explanation his terminology). CAs are important for several fundamental metabolic processes, from CO2 transport to acid-base balance, because they catalyze the reversible hydration of CO2. In our opinion, CAs might play a significant role in the substratum dissolution, because their secretion in the sites of active growth would significantly intensify the chemical activity of respiratory CO2, whereas their presence in the hyphae would favour lipogenesis, that might explain the high content in triacylglycerols typical of endolithic lichens. We hope that this answer satisfied your curiosity. Do not hesitate to contact one of us for further information: as you can imagine, part of this text was taken from a manuscript (in prep.)! Ciao ciao, Mauro Tretiach (Trieste), Paolo Modenesi (Genova) and Rosanna Piervittori (Torino), ITALY At 18.05 12/04/07, Scott LaGreca wrote: Dear All, I am helping our Exhibitions team with a new "Antarctica" exhibition they're working on and they want to include some Antarctic lichen specimens, especially "endolithic lichens". Can anyone on this list please provide me with a definition of "endolithic lichen"? I assume it means a lichen that has its entire vegetative thallus within a saxicolous substrate--and only its fruiting bodies can be seen above the surface of the rock. Of course this would mean that many common species of lichen, e.g. Porpidia albocaerulescens, are endolithic lichens. True? Another question which the team has asked me: Are endolithic lichens only found in Antarctica? I told them "'no, but the endolithic habit is a common form for Antarctic lichens in really harsh parts of the continent." Can anyone comment please? Any help would be appreciated. Thanks very much, Scott ____________________________ Scott LaGreca, PhD Curator of Lichens Herbarium, Botany Department the Natural History Museum London SW7 5BD United Kingdom telephone: [+44] 20 7942 5250 fax: [+44] 20 7942 5529 ____________________________ Prof. Mauro Tretiach Dipartimento di Biologia Università di Trieste Via L. Giorgieri, 10 I-34127 Trieste Italy tel. +39 040 558 3886 fax +39 040 575079 mob. 329 0688968